Radioprotective effect of melatonin in reducing oxidative stress in rat lenses

Shirazi, A. and Haddadi, G.H. and Asadi-Amoli, F. and Sakhaee, S. and Ghazi-Khansari, M. and Avand, A. (2011) Radioprotective effect of melatonin in reducing oxidative stress in rat lenses. Cell Journal, 13 (2). pp. 79-82.

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Objective: Ocular morbidity is widely observed when radiotherapy includes the orbit. Oxidative stress generated by irradiation is responsible for this complication. In different studies, it has been shown that melatonin has antioxidative properties and a radioprotective role. The aim of this study was to evaluate the antioxidant role of melatonin against radiation-induced oxidative injury in rats' lenses after total cranial irradiation. Materials and Methods: Thirty-six adult female Sprague-Dawley rats were divided into six groups. Group I was the control group, group II only received total cranial gamma irradiation of 5 Gy, group III was exposed as the second group but at the dose of 8 Gy, group IV received 30 mg/kg melatonin 30 minutes prior to radiation plus total cranial irradiation of 5 Gy plus 5 mg/kg melatonin daily through intraperitoneal injection for ten days after irradiation, group V was treated similar to the fourth group, i.e. received irradiation plus melatonin, but at the dose of 8 Gy, and group VI only received melatonin (30 mg/kg on the first day and 5 mg/kg on the following days). Ten days after irradiation, all rats were sacrificed and their eyes were enucleated to measure the biochemical parameters i.e. malondialdehyde (MDA) and glutathione (GSH). Results: The levels of MDA in rat lenses increased and the levels of glutathione in lenses decreased after gamma ray irradiation but these parameters were still within normal limits in rats that received melatonin. Conclusion: It could be concluded that melatonin is useful in preventing radiation-induced oxidative injury due to its antioxidative and free radical scavenging properties.

Item Type: Article
Additional Information: cited By 13
Uncontrolled Keywords: free radical; glutathione; malonaldehyde; melatonin, animal experiment; animal tissue; antioxidant activity; article; biochemistry; brain radiation; concentration (parameters); controlled study; enzyme activity; eye disease; female; gamma irradiation; lens; nonhuman; oxidative stress; radiation dose; radiation exposure; radiation protection; rat; tissue homogenate; treatment duration
Divisions: School of Medicine
Depositing User: Unnamed user with email
Date Deposited: 09 Mar 2017 21:36
Last Modified: 09 Mar 2017 21:36

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