The effect of the methanol extract of galium mite on the cellular immunity and antibody synthesis

Amirghofran, Z. and Javidnia, K. and Bahmani, M. and Azadmehr, A. and Esmaeilbeig, M. (2011) The effect of the methanol extract of galium mite on the cellular immunity and antibody synthesis. Journal of Immunoassay and Immunochemistry, 32 (3). pp. 157-169.

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Abstract

In the present study, the immunomodulatory effects of Galium mite, a native herb used for the treatment of inflammation in Iranian traditional medicine, was investigated. The methanolic extract of the plant was prepared and examined for in vivo cell-mediated and humoral immunity against antigen in mice. Galium mite stimulated delayed type hypersensitivity at lower concentrations and inhibited the reaction at higher ones (p0.05). A dose-related decrease in primary and secondary antibody titer was observed in mice treated with the extract (p0.006). The extract at higher concentrations significantly reduced the proliferation of human-activated lymphocytes (p0.001). Cell cycle analysis on human lymphocytes treated with the extract showed an increase in the number of cells in sub-G1 region, indicating the ability of the extract to induce apoptosis in these cells. Induction of apoptosis was confirmed by DNA laddering on gel electrophoresis. In conclusion, G. mite has the ability to modulate cellular and humoral immune responses to the antigenic challenge and affect the rate of cell proliferation due to induction of apoptosis in the lymphocytes. Copyright © Taylor & Francis Group, LLC.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: Galium mite extract; immunomodulating agent; levamisole; methanol; plant extract; unclassified drug, animal experiment; antibody production; antibody titer; apoptosis; article; cell cycle G1 phase; cellular immunity; controlled study; delayed hypersensitivity; DNA fragmentation; flow cytometry; Galium; human; human cell; humoral immunity; Iran; lymphocyte proliferation; male; mouse; nonhuman; priority journal; traditional medicine, Animals; Antibodies; Apoptosis; Cell Proliferation; Galium; Humans; Hypersensitivity, Delayed; Immunity, Cellular; Immunity, Humoral; Injections, Subcutaneous; Lymphocytes; Male; Methanol; Mice; Plant Extracts; Sheep
Divisions: School of Medicine
Depositing User: Unnamed user with email eprints@fums.ac.ir
Date Deposited: 09 Mar 2017 21:35
Last Modified: 09 Mar 2017 21:35
URI: http://eprints.fums.ac.ir/id/eprint/253

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