A multiplex real-time PCR technique for simultaneous detection of Listeria monocytogenes and Streptococcus agalactiae among asymptomatic pregnant women

Ghasemian, Abdolmajid and Nojoomi, Farshad and Abhari, Mina Heidari and Mehrabadi, Jalil Fallah A multiplex real-time PCR technique for simultaneous detection of Listeria monocytogenes and Streptococcus agalactiae among asymptomatic pregnant women. REVIEWS IN MEDICAL MICROBIOLOGY, 29 (3). pp. 136-139.

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Abstract

Objective: Listeria monocytogenes and Streptococcus agalactiae are important causes of urogenital and infantile infections. This study was performed to design a multiplex-real- time PCR technique for simultaneous detection of L. monocytogenes and S. agalactiae. Methods: A total of 233 vaginal samples from pregnant patients (35-37 weeks of pregnancy) were collected. For L. monocytogenes, after swab lawn the plates were incubated at 37 degrees C for up to 48 h and after bacterial growth, 50 ml of broth medium was inoculated to blood agar. The isolates were also identified by phenotypic tests. The PCR and real-time PCR techniques were performed to amplify the hly and cfo genes from L. monocytogenes and S. agalactiae, respectively. Results: In the PCR technique, 17 (7.29%) and 25 (10.72%) isolates were identified as L. monocytogenes and S. agalactiae by amplification of hly and cfo genes, respectively, and similarly in the culture method 16 (6.68%) and 23 (9.87%) isolates were obtained and identified, respectively. By the real-time PCR, L. monocytogenes and S. agalactiae were detected in 24 (10.30%) and 34 (14.60%) samples, respectively. Real-time PCR demonstrated the highest the sensitivity and specificity results. Conclusion: The real-time PCR was the most advantageous method due to its higher sensitivity and specificity and rapidity in results, especially among asymptomatic patients. L. monocytogenes and S. agalactiae infections need to be diagnosed rapidly because of complications occurred among infants and asymptomatic pregnant women. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.

Item Type: Article
Subjects: QW Microbiology and Immunology
Depositing User: Unnamed user with email eprints@fums.ac.ir
Date Deposited: 24 Jul 2019 07:10
Last Modified: 24 Jul 2019 07:10
URI: http://eprints.fums.ac.ir/id/eprint/2383

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