Histomorphometrical study of the effect of platelet-rich plasma therapy on the damage caused by the Torsion-Detorsion testicular in mice

aghaeiborashan, fatemeh and Shahrooz, rasoul and Ahmadi, abbas and MazaheriKhamene, ramin and Imani, mehdi (2018) Histomorphometrical study of the effect of platelet-rich plasma therapy on the damage caused by the Torsion-Detorsion testicular in mice. Journal of Fasa University of Medical Sciences, 8 (3). pp. 881-889.

[img] Text
1.pdf

Download (971kB)
Official URL: http://journal.fums.ac.ir/article-1-1550-en.html

Abstract

Background & Objectives: Testicular Torsion is one of the emergency cases which is caused by twisting of the spermatic cord; Detorsion leads to the recirculation of blood and ultimately, more damage occurs in testis. Platelet-Rich plasma (PRP) contains several growth factors and cytokines that can restore tissue and rebuild cells, so in this study, PRP was used to evaluate its effects. Materials & Methods: Twenty-eight adult male mice were randomly divided into three equal groups (n = 6). The first group, is the healthy control, without testicular twisting. Group II: Torsion Detorsion (TD) + PBS (phosphate-buffer-solution) group, Group III: The TD + PRP group. In these two groups spermatic cord were twisted for one hour, and after detorsion, PBS and PRP were injected into the rete testis, respectively. 35 days after surgery, left testis was sampled for histomorphometrical examinations. Obtained data were statistically compared by ANOVA and post hoc-Tukey test (P <0.05). Results: Obtained data from the mean thickness of germinal epithelium, diameter of seminiferous tubules (P<0.01), and, the spermatogenesis indices (P<0.001) were decreased significantly in TD+ PBS group. The thickness of the testis capsule and the lumen of seminiferous tubules showed a significant increase (P<0.01). While, PRP significantly improved these parameters (P<0.01). Conclusion: Testicular TD causes many impacts and disorganization in the male reproductive system, and resulting infertility. It seems that, PRP due to possession ample of growth factors, able to relatively improve the undesirable effects of TD.  

Item Type: Article
Uncontrolled Keywords: Testicular Torsion-Detorsion, Platelet Rich Plasma, Histomorphometry, Testis, Mice
Depositing User: Unnamed user with email eprints@fums.ac.ir
Date Deposited: 04 Mar 2019 09:24
Last Modified: 04 Mar 2019 09:24
URI: http://eprints.fums.ac.ir/id/eprint/2215

Actions (login required)

View Item View Item