Evaluation of immunomodulatory effects of mesenchymal stem cells soluble factors on miR-155 and miR-23b expression in mice dendritic cells

Karachi, A. and Fazeli, M. and Karimi, M.H. and Geramizadeh, B. and Moravej, A. and Ebrahimnezhad, S. and Afshari, A. (2015) Evaluation of immunomodulatory effects of mesenchymal stem cells soluble factors on miR-155 and miR-23b expression in mice dendritic cells. Immunological Investigations, 44 (5). pp. 427-437.

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Abstract

Mesenchymal stem cells (MSCs) can modulate dendritic cells (DCs) activation and induce tolerogenic characteristics in DCs. All mechanisms involved in MSCs-induced tolerogenic DCs are not fully understood. MicroRNAs (miRs) play important role in maturation and function of DCs. In this study, we investigated the effects of MSCs culture supernatant (C.S.) on expression of miR-155 and miR-23b in mice DCs. BALB/c mice spleens were used for DCs isolation. MSCs were isolated from the mice bone marrow and cultured in DMEM media. When MSCs expanded to sixth passage, C.S. was collected after 12, 24 and 48h. Quantitative polymerase chain reaction (QPCR) was used to determine the expression of miR-155 and miR-23b in DCs treated with C.S. after 6 and 12h. Secretion of IL-23 and TGF- β were detected in DCs treated with C.S. by ELISA after 24h. miR-23b expression was significantly increased in DCs treated with 12h C.S. for 12h compared to negative controls. miR-155 expression did not change in DCs treated with C.S. after 6 and 12h. miR-23b expression was significantly increased in DCs treated with 12h C.S. for 12h, compared to those treated with C.S. for 6h. Similarly, miR-23b expression was increased in DCs treated with 24h C.S. for 12h when compared to those treated for 6h. Production of TGF-β and IL-23 were not influenced by C.S. In conclusion, miR-23b is considered to be one of the mechanisms involved in tolerogenic DCs induction by C.S. in a time-dependent manner. © 2015 Informa Healthcare USA, Inc.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: interleukin 23; microRNA; microRNA 155; microRNA 23b; transforming growth factor beta; unclassified drug; culture medium; cytokine; immunologic factor; microRNA; Mirn155 microRNA, mouse; Mirn23b microRNA, mouse, animal cell; Article; bone marrow derived mesenchymal stem cell; cell activation; cell maturation; controlled study; cytokine release; dendritic cell; enzyme linked immunosorbent assay; gene expression; immunological tolerance; immunomodulation; mouse; nonhuman; polymerase chain reaction; priority journal; quantitative analysis; spleen; stem cell expansion; supernatant; adipogenesis; animal; Bagg albino mouse; biosynthesis; bone development; cell culture; cell differentiation; culture medium; cytology; dendritic cell; drug effects; gene expression regulation; genetics; germfree animal; isolation and purification; mesenchymal stroma cell; metabolism; pharmacology; secretion (process); time, Adipogenesis; Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytokines; Dendritic Cells; Gene Expression Regulation; Immunologic Factors; Mesenchymal Stromal Cells; Mice; Mice, Inbred BALB C; MicroRNAs; Osteogenesis; Specific Pathogen-Free Organisms; Spleen; Time Factors
Divisions: School of Medicine
Depositing User: Unnamed user with email eprints@fums.ac.ir
Date Deposited: 10 Mar 2017 17:13
Last Modified: 10 Mar 2017 17:13
URI: http://eprints.fums.ac.ir/id/eprint/152

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